QuantPrime - a flexible primer design tool for high-throughput qPCR

QuantPrime is an intuitive and user-friendly, fully automated tool for primer pair design in small- to large-scale real-time reverse transcription qPCR (also known as realtime qRT-PCR or RT-qPCR) analyses. QuantPrime can be used on the website or on a local computer (contact us for getting a copy); it offers design and specificity checking with highly customizable parameters and is ready to use with most publicly available eukaryotic transcriptomes, including all higher eukaryote model organisms and important plant crops, while benefiting from exon-intron border and splice variant information in available genome annotations. Experimental results with the model plant Arabidopsis thaliana, the crop Hordeum vulgare (barley) and the model green alga Chlamydomonas reinhardtii show success rates of designed primer pairs exceeding 96 %. [more...]


Permanently, upon request:
Database updates.
Jul 3rd-6th 2011
Major database update: 597 new annotations added or updated from Ensembl, EnsemblGenomes, Phytozome, NCBI RefSeq and UniGene. [more...]

Dec 8th 2011
The Mt3.5 v3 and v4 annotations for Medicago truncatula were added.

May 10th 2011
The ITAG annotations (release 1, 2 and 2.3) for tomato (Solanum lycopersicum) were added.

Please cite as follows when using primers designed with QuantPrime in published works:
Samuel Arvidsson, Miroslaw Kwasniewski, Diego M. Riano-Pachon and Bernd Mueller-Roeber: QuantPrime - a flexible tool for reliable high-throughput primer design for quantitative PCR. BMC Bioinformatics 2008, 9:465

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