QuantPrime - a flexible primer design tool for high-throughput qPCR
QuantPrime is an intuitive and user-friendly, fully automated tool for primer pair design in small-
to large-scale real-time reverse transcription qPCR (also known as realtime qRT-PCR or RT-qPCR) analyses. QuantPrime can be used on the website or on a local computer
(contact us for getting a copy); it offers design and specificity
checking with highly customizable parameters and is ready to use with most publicly available eukaryotic
transcriptomes, including all higher eukaryote model organisms and important plant crops, while benefiting
from exon-intron border and splice variant information in available genome annotations. Experimental results
with the model plant Arabidopsis thaliana, the crop Hordeum vulgare (barley) and the model green alga
Chlamydomonas reinhardtii show success rates of designed primer pairs exceeding 96 %.
Permanently, upon request:
Jul 3rd-6th 2011
Major database update: 597 new annotations added or updated from Ensembl, EnsemblGenomes, Phytozome, NCBI RefSeq and UniGene. [more...]
Dec 8th 2011
The Mt3.5 v3 and v4 annotations for Medicago truncatula were added.
May 10th 2011
The ITAG annotations (release 1, 2 and 2.3) for tomato (Solanum lycopersicum) were added.