QuantPrime is an intuitive and user-friendly, fully automated tool for primer pair design in small- to large-scale real-time reverse transcription qPCR (also known as realtime qRT-PCR or RT-qPCR) analyses. QuantPrime can be used on the website or on a local computer (contact us for getting a copy); it offers design and specificity checking with highly customizable parameters and is ready to use with most publicly available eukaryotic transcriptomes, including all higher eukaryote model organisms and important plant crops, while benefiting from exon-intron border and splice variant information in available genome annotations. Experimental results with the model plant Arabidopsis thaliana, the crop Hordeum vulgare (barley) and the model green alga Chlamydomonas reinhardtii show success rates of designed primer pairs exceeding 96 %. [more...]

Please cite as follows when using primers designed with QuantPrime in published works:
Samuel Arvidsson, Miroslaw Kwasniewski, Diego M. Riano-Pachon and Bernd Mueller-Roeber: QuantPrime - a flexible tool for reliable high-throughput primer design for quantitative PCR. BMC Bioinformatics 2008, 9:465